Part:BBa_K798000

450bp overlong telomere repeats of Saccharomyces cerevisiae

This part is a 450bp overlong telomere repeats of Saccharomy cescerevisiae(its general length is about 300bp). According to some researches before, the overlong telomere in the yeast will decrease by 2-3bp per generation. Thus, we can insert some key regulation sequence (but the sequence has to be very small, <100bp) in the overlong part of the telomere sequence. After recombination into the yeast chromosome, the telomere begins to decrease and about 70 generations later the key sequence will be cut off which can have an effect on the regulation. Obviously, the main advantage of the design is that we can change the regulation procedurally and predictably.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 465
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 1
  Illegal SpeI site found at 466
  Illegal PstI site found at 480
  Illegal NotI site found at 7
  Illegal NotI site found at 473
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 1
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 1
  Illegal suffix found in sequence at 466
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 1
  Illegal XbaI site found at 16
  Illegal SpeI site found at 466
  Illegal PstI site found at 480
  
• 1000
  COMPATIBLE WITH RFC[1000]