Plasmid_Backbone

Part:BBa_K797016

Designed by: Komoto Yusuke   Group: iGEM12_Kyoto   (2012-09-26)


Plasmid Backbone including M-13 primer region

Plasmid Backbone that contains M13-fwd

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 136
    Illegal suffix found at 158
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 136
    Illegal SpeI site found at 159
    Illegal PstI site found at 173
    Illegal NotI site found at 142
    Illegal NotI site found at 166
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 136
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 136
    Illegal suffix found at 159
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 136
    Plasmid lacks a suffix.
    Illegal XbaI site found at 151
    Illegal SpeI site found at 159
    Illegal PstI site found at 173
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1163


[edit]
Categories
Parameters
None