Project

Part:BBa_K787004

Designed by: Anish Vaghela   Group: iGEM12_Rutgers   (2012-10-02)


pBBR1-aceE.aceF.lpd

lac\ promoter, aceE, aceF, lpd

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2418
    Illegal XbaI site found at 6434
    Illegal PstI site found at 635
    Illegal PstI site found at 2646
    Illegal PstI site found at 3558
    Illegal PstI site found at 3777
    Illegal PstI site found at 5943
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2418
    Illegal NheI site found at 1844
    Illegal PstI site found at 635
    Illegal PstI site found at 2646
    Illegal PstI site found at 3558
    Illegal PstI site found at 3777
    Illegal PstI site found at 5943
    Illegal NotI site found at 10784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2418
    Illegal BglII site found at 422
    Illegal BglII site found at 2929
    Illegal BglII site found at 3337
    Illegal BamHI site found at 1889
    Illegal XhoI site found at 1838
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2418
    Illegal XbaI site found at 6434
    Illegal PstI site found at 635
    Illegal PstI site found at 2646
    Illegal PstI site found at 3558
    Illegal PstI site found at 3777
    Illegal PstI site found at 5943
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2418
    Illegal XbaI site found at 6434
    Illegal PstI site found at 635
    Illegal PstI site found at 2646
    Illegal PstI site found at 3558
    Illegal PstI site found at 3777
    Illegal PstI site found at 5943
    Illegal NgoMIV site found at 1086
    Illegal NgoMIV site found at 1369
    Illegal NgoMIV site found at 3959
    Illegal NgoMIV site found at 5428
    Illegal NgoMIV site found at 8512
    Illegal AgeI site found at 2670
    Illegal AgeI site found at 6749
    Illegal AgeI site found at 8352
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 935
    Illegal SapI.rc site found at 1145
    Illegal SapI.rc site found at 3201
    Illegal SapI.rc site found at 3321


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