Coding

Part:BBa_K784052

Designed by: Asaf Blumental   Group: iGEM12_Technion   (2012-09-26)


T7*(T3) RNAP double stop codon

This part includes T7*(T3) RNA Polymerase gene. This is orthogonal polymerases, based on T7 wt RNAP, that recognizes specifically a suitable promoter- pT3. The T7*(T3) RNA Polymerase was donated us by Chris Voight lab (see source). The T7*(T3) RNA Polymerase is less toxic to host cells, than the T7 wt. In order to achieve reduced levels of toxicity, Chris and his team had added N-terminal degradation tag to the T7 wt polymerase to reduce its concentration in host E.coli cell and controlled the RNAP expression using a weak ribosome-binding site and ATG start codon. The specificity of the T7*(T3) to pT3 was achieved by engineering a DNA binding domain within the RNA polymerase.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None