Composite

Part:BBa_K784045

Designed by: Inbal Vaknin   Group: iGEM12_Technion   (2012-09-25)


Mutant T7 RNAP + BBa_B0015 terminator

The part was made by cloning the T7 RNAP gene to the BioBrick BBa_B0015, which includes double terminator (B0010-B0012). This part is present in the plasmid pSB1AK3. The purpose of this part is that you can clone to it any gene you want to be combined with it: promoter, reporter gene etc..

Note: the biobrick prefix has been altered to deletion of one bp downstream to the XbaI site. This was due to wrong primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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