Part:BBa_K784041
pTetO+ mCherry (FP)
This part includes 2 inducible promoters of pTetO (activated by tetracycline), for reducing the leakiness of this promoter, and a red fluorescence protein (mCherry). This part was donated to us by Roee Amit, and was sequence confirmed by him. The part is present in plasmid pSB1C3, with the standard prefix and suffix, but the sequence of the part contains EcoRI and PstI restriction sites, so the optimal solution will be using this part in Gibson assembly reaction. This part places mCherry downstream to the pTetO promoter. Reported activities of the promoters are given in strain Top10 grown in LB media. The activity of this promoter in this bacteria strain is constitutive and the fluorescence measure results can be seen at the "results for this part" section. This part can be used for inducible/constitutive expression (depends on the bacteria's strain) of the FP, and can be combined with other genes. Cloning this part was done by amplifying the promoter and the FP together, adding XbaI site in the 5' and SpeI in the 3' ends, for cloning it to the registry plasmids.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 75
Illegal PstI site found at 453 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 75
Illegal PstI site found at 453 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 75
Illegal BglII site found at 816
Illegal BamHI site found at 840 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 75
Illegal PstI site found at 453 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 75
Illegal PstI site found at 453 - 1000COMPATIBLE WITH RFC[1000]
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