Composite

Part:BBa_K784034

Designed by: Noa Katz   Group: iGEM12_Technion   (2012-09-18)


pSp6_xyIE reporter gene

This part's purpose is to testify to the presence of the Sp6 RNA Polymerase by the xyIE reporter gene from iGEM2006_Edinburgh (https://parts.igem.org/Part:BBa_J33204). It consists of an RNA Sp6 promoter, an RBS, a reporter gene- xyIE, and a T7 terminator. The assay for xyIE is very simple, refer to the xyIE basic part for instructions.

The xyIE+RBS part is between XhoI and XmaI restriction sites, and therefore can be excised by these enzymes.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 29
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 371
    Illegal NgoMIV site found at 543
    Illegal AgeI site found at 894
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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