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Part:BBa_K784015

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)

Phage Lambda fragment 1 - head proteins

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
Each fragment was inserted into pSB1C3_MCS plasmid (BBa_K784023), which was designed mainly for this purpose. The MCS contain PacI and SpeI recognition sites, which does not appear at the phage genome.
More information is available [http://2012.igem.org/Team:Technion/Project/Phage here].
Fragment 1 contains the phages head proteins, which together with fragment 2 (BBa_K784016), 3 (Part:BBa_K784017) and 4 (BBa_K784018) create the phage envelope.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 436
    Illegal BamHI site found at 5526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2577
    Illegal PstI site found at 2841
    Illegal PstI site found at 3646
    Illegal PstI site found at 3661
    Illegal PstI site found at 3877
    Illegal PstI site found at 4391
    Illegal PstI site found at 4730
    Illegal AgeI site found at 4751
    Illegal AgeI site found at 4900
    Illegal AgeI site found at 6582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2418
    Illegal SapI.rc site found at 6510


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