RBS
Part:BBa_K784012
Designed by: Ilya Vainberg Slutskin Group: iGEM12_Technion (2012-09-16)
Spacer2 + RBS from theophylline riboswitch
This part has been created by deleting the aptamer and the additional 5' UTR regions of the theophylline riboswitch using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the theophylline riboswitch remains.
This part is the result of an insertion mutation to BBa_K784007.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |