RBS

Part:BBa_K784012

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-16)


Spacer2 + RBS from theophylline riboswitch

This part has been created by deleting the aptamer and the additional 5' UTR regions of the theophylline riboswitch using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the theophylline riboswitch remains.

This part is the result of an insertion mutation to BBa_K784007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None