Plasmid

Part:BBa_K783063

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)


Level 1 MoClo Destination Vector with EF

This is a Level 1 MoClo destination vector with flanking sites E on the 5' side and site F on the 3' side of the alpha fragment of lacZ.

The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None