Coding

Part:BBa_K783053

Designed by: Traci Haddock   Group: iGEM12_BostonU   (2012-10-02)

This is a MoClo converted version of BBa_E0040

This is a MoClo converted version of BBa_E0040, which is a green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP.

It has two MoClo fusion sites flanking it: 5' C [part] D 3'

The fusion site letters refer to 4bp fusion sites: A = GGAG; E = GCTT; B = TACT; F = CGCT; C = AATG; G = TGCC; D = AGGT; H = ACTA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None