Composite

Part:BBa_K781007

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-03)


[R0010][B0034] - XylE-FliC Deletion Chimera

This is an XylE deletion variant of the flagellin monomer. The enzyme XylE that degrades catechol was incorporated into the E. coli flagellin by replacing the variable domain.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2130
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1118
    Illegal NgoMIV site found at 1290
    Illegal AgeI site found at 529
    Illegal AgeI site found at 1641
    Illegal AgeI site found at 1726
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None