Generator

Part:BBa_K780004

Designed by: iGEM 2012 Warsaw Team   Group: iGEM12_Warsaw   (2012-09-26)

RBS+GFP for expression in Bacillus subtilis

It is succesfully joined B.subtilis RBS with GFP reporter protein. Can be used to measure strenght of B.subtilis promoters. In order to work with it you must first add appropriate terminator at the end. We recommend BBa_K780000 - a terminator designed for use with B.subtilis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 666

Other notes

Was proven to work in E.coli as well but with much lower efficiency.

Measurement

Strenght: 10.13% of BBa_B0032 RBS (measured in E.coli)


[edit]
Categories
//chassis/prokaryote/bsubtilis
//classic/generator/uncategorized
//function/reporter/fluorescence
Parameters
None