Designed by: Nikodem Latocha Group: iGEM12_Warsaw (2012-09-26)
Terminator for Bacillus subtilis
- Our terminator is based on strong, Rho-independent terminator of gyrase gene, which is type II topoisomerase (EC 220.127.116.11). DNA gyrase uses the energy of ATP hydrolysis to introduce negative supercoils or relaxes positive supercoils.
- Negative DNA supercoiling is essential in vivo to compact the genome, relieve torsional strain during replication, and promote local melting for vital processes such as transcript initation by RNA polymerase.
- Gyrase gene is a housekeeping gene and because of that it must have efficient terminator.