Part:BBa_K779403

U6-TetO-FF4 MammoBlock

The U6-TetO-FF4 part encodes conditional expression of the short RNA sequence FF4. The mammalian RNA Pol III U6 promoter incorporates tetracycline-responsive elements that allow for tightly regulated RNA expression under specific conditions. In the presence of the Tet repressor (TetR), two Tet operator (TetO) sites become bound by the TetR and prevent RNA Pol III from binding, subsequently inhibiting transcription of the downstream sequence. In the absence of TetR, RNA transcription proceeds normally. The short FF4 RNA sequence can be used as an RNA interference mechanism through its binding to a complementary region present in the transcript of another gene, thus inhibiting translation of that sequence and silencing expression of that gene. Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 478
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 478
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 478
  Illegal XhoI site found at 375
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 478
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 478
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 248