Reporter
Part:BBa_K779304
Designed by: Giulio Alighieri Group: iGEM12_MIT (2012-09-29)
mKate MammoBlock
A mammalian-codon optimized red fluorescent protein. This protein is a significantly engineered reporter protein that, unlikely many fluorescent proteins, is monomeric and therefore ideal for fusion constructs.
200,000 cells were transiently transfected with 500 ng of Hef1A:mKate using Lipofectamine 2000. Images were taken on an Evos microscope at 10X. The left image is brightfield. The right is in the Texas-Red channel. Very high transfection efficiency and mKate expression levels can be observed when mKate is driven by the constitutive promoter Hef1A (BBa_K779200).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 641
Illegal SapI.rc site found at 23
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Categories
Parameters
n/a | mKate MammoBlock |