Part:BBa_K778011

hycAp-GFPgenerator-plac-FhlA363generator

You can "enhance" and "see" H2 production with this device.

hycAp-GFPgenerator-plac-FhlA363generator

GFP is a reportor of H2 production (strictly speaking,reporter of hycAp activation Part:BBa K778000 (hycAp is the promotor of formate hydrogen lyase of genome)).

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)). fhlA-E363G has one point mutation (A to G, glutamic acid to glycine). This mutation increased H2 production under the conditions in the previous research (Viviana et.al.2009). However, in our research (team UT-Tokyo 2012[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli]), this mutation did not increase H2 production.

Detailed Information :[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli/System]

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 3266
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2545
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 828