Generator
RBS-fhlA-d

Part:BBa_K778004

Designed by: Kohta Toshimitsu & Takahiro Yamada   Group: iGEM12_UT-Tokyo   (2012-09-23)

RBS-fhlA-d.term

FhlA generator without promoter (RBS-fhlA-d.term)

FhlA is the trnascriptional activator of hycAp Part:BBa K778000 . E.coli overexpressing FhlA produce H2 (since hycAp is the promoter of FHL(formate hydrogen lyase)).

UT Tokyo H2 1001+eg.png

(The full construct in this experiment is Part:BBa K778007 and the modified fhlA(E363G) part is Part:BBa K778002.)

Detailed Information : Our wiki(UT-Tokyo 2012)[http://2012.igem.org/Team:UT-Tokyo/Project/H2_E.coli] The results shown on the wiki are different from the latest result on this parts.igem page.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2115
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1394
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None