Coding

Part:BBa_K777000

Designed by: Bianca Genenncher   Group: iGEM12_Goettingen   (2012-09-11)

Tar receptor

Fig. 1: Tar chemoreceptor 3D structure.

The transmembrane chemoreceptor Tar of E. coli mediates chemotaxis towards aspartate and exists as a functional homodimer.

Figure 1 represents the non quikchanged Tar chemoreceptor binding aspartate within its binding site and was constructed using the software PYMOL (PDB ID:1WAT).









Usage and Biology

This part can be used for chemotaxis assays.

  • Here is the complete sequence of this part as an ApE file.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1282
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 111


Fig. 2: Plasmid map of K777000 in pSB1C3


[edit]
Categories
//chassis/prokaryote/ecoli
//promoter/anderson
//regulation/constitutive
Parameters
biologyEscherichia coli
chassisE. coli
ligandsAspartate
positive_regulatorsJ23100 constitutive promoter
proteinTar