Part:BBa_K775013

pRSII415

This shuttle vector is for use in the budding yeast Saccharomyces cerevisiae and the bacterium E. coli. This vector contains the f1 multiplecopy origin of replication for E. Coli and the CEN6/ARSH4 yeast origin of replication. For E. coli it has the ampicilline resistance and for yeast the auxotrophic selectable marker LEU2. The pRS415-II plasmid contains, in contrast to the previous pRS series, no illegal restriction site (EcorI, PstI, XbaI or SpeI) in the selectable marker site. The multiple cloning site (MCS) does contain these restriction sites, however the order of the restriction sites is different in comparison to the backbones that are compatible with the standard assembly method for biological parts (EPSX instead of EXSP).

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 3164
  Illegal XbaI site found at 3194
  Illegal SpeI site found at 3188
  Illegal PstI site found at 3170
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 3164
  Illegal SpeI site found at 3188
  Illegal PstI site found at 3170
  Illegal NotI site found at 3200
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 3164
  Illegal BamHI site found at 3182
  Illegal XhoI site found at 3131
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 3164
  Illegal XbaI site found at 3194
  Illegal SpeI site found at 3188
  Illegal PstI site found at 3170
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 3164
  Illegal XbaI site found at 3194
  Illegal SpeI site found at 3188
  Illegal PstI site found at 3170
  Illegal NgoMIV site found at 2792
  Illegal AgeI site found at 2406
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 4576
  Illegal SapI site found at 3493
  Illegal SapI site found at 5597