Coding

Part:BBa_K775001

Designed by: Sietske Grijseels   Group: iGEM12_TU-Delft   (2012-09-05)


RI7-Olfr154 Isoamylacetate chimeric GPCR

BBa_K775001 encodes a chimeric isoamylacetate-sensing G-protein coupled receptor which can be functionally expressed in S. cerevisiae. This chimeric receptor is composed of the ligand-binding domain of the mouse Olfr154, flanked by the N- and C- terminals of the rat OR RI7.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 906


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Categories
Parameters
None