Generator

Part:BBa_K772102

Designed by: Mustafa Elitok   Group: iGEM12_Fatih   (2012-09-26)

Halorhodopsin ready-to-use system

Halorhodopsin is an inward-directed light-driven chloride ion pump originating from Halobacterium. It utilizes light to pump chloride ions against chloride concentration difference into cells from the environment.

We added a constitutive working and inducible pLac promoter in the upstream region of the part in order to use it in our design easily.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 608
    Illegal AgeI site found at 334
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 544


[edit]
Categories
//cds/membrane/channel
//chassis/prokaryote/ecoli
//function/sensor/light
Parameters
biologyHalobacterium
device_typeIon Channel
functionLight-driven ion pumping
proteinHalorhodopsin