Part:BBa_K772004
AHL regulated PoPS generator: Las receiver device
Quorum sensing is the mechanism of communication between bacteria via synthesizing and detecting chemical compounds in the media. With these mediators, the promoters and their yield can be tuned or regulated. This part represents an alternative quorum sensing receiver device, which is able to generate a PoPS response in the presence of 3OHSL6 produced by LasI, instead of BBa_F2620. This will allow iGEMers to establish communications between different bacteria colonies and it makes simple to generate easy regulated complex systems. This part is assembled with Las promoter system to improve the current quorum sensing devices and parts which commonly base on Lux promoter system. pLas promoter works in the presence of both 3OHSL6 and LasR protein.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1021
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 400
Illegal AgeI site found at 597 - 1000COMPATIBLE WITH RFC[1000]
//chassis/prokaryote/ecoli
//classic/regulatory/other
//classic/signalling/receiver
//function/regulation/transcriptional
regulator
| chassis | E.coli TOP10 strain |
| device_type | Receiver/PoPS Generator |
| function | Quorum sensing |
| input_s | LasR, 3OC12HSL |
| output_s | PoPS |
| positive_regulators | LasR, 3OC12HSL |
| receiver | pLas |
| signalling_molecule | LasR |