Device

Part:BBa_K771405

Designed by: WU Yuqi   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-25)

split EGFP 2 with Membrane Anchor 5(without VVD)



Membrane anchor 5 without VVD, which consists of GBD ligand(BBa_K771106) and membrane protein Lgt(BBa_K771102). Membrane Anchor 5 (with and without VVD) is proved to constitutively dimerize with Membrane Anchor [BBa_K771004].


We proved the constitutive interaction between membrane anchor 4 and 5 by fusing split EGFP to membrane anchor 4 and membrane anchor 5 without VVD.

Membrane Anchor 4 and Membrane Anchor 5 without VVD

12SJTU splitegfp45.png
12SJTU Standard GFP 2.jpg

We fused split EGFP 1 and 2 to Membrane Anchor 4 and Membrane Anchor 5 without VVD to test whether Membrane Anchor 4 and Membrane Anchor 5 without VVD could constitutively aggregate. Proteins in control group are not expected to aggregate like in experimental group.We coexpressed proteins in experimental group and control group respectively in E.coli. After induction for 6 hours, bacteria samples were taken for fluorescence observationThe result shows that Membrane Anchor 4 and Membrane Anchor 5 without VVD could constitutively aggregate through GBD domain and ligand.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 884
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 505


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Categories
Parameters
n/asplit EGFP 2 with Membrane Anchor 5(without VVD)