Part:BBa_K771203
VioE with Membrane Anchor 4
This part is composed with two parts, the Membrane Anchor 4 and Violacein synthetic pathway enzyme vioE. The enzyme can be aggregated with other membrane anchors, composing the Membrane Magic system.This part is costructed without MS2(BBa_K771112). For more information, please visit 2012 SJTU-BioX-Shanghai wiki
Usage and Biology
In our Violacein Membrane Rudder System, VioA, VioB, VioE and VioC constitutively aggregate. The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA.
When blue light is present, VioD with Membrane Anchor 6 would aggregate with complex of VioA,B,C and E, making violacein the dominnant final product.But if you turn the light off, VioD with Membrane Anchor would dissociate with VioA,B,C and E. Thus the biosynthetic pathway for deoxyviolacein is switched on.
Related Parts
VioA with Membrane Anchor 2 (BBa_K771201)
VioB with Membrane Anchor 3 (BBa_K771202)
VioE with Membrane Anchor 4 (BBa_K771203)
VioC with Membrane Anchor 5 (BBa_K771204)
VioC with Membrane Anchor 5 (BBa_K771205)
VioD with Membrane Anchor 6 (BBa_K771206)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 115
Illegal AgeI site found at 1168 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 345
Illegal BsaI site found at 789
| n/a | VioE with Membrane Anchor 4 |