Part:BBa_K771201

VioA with Membrane Anchor 2(without MS2)

This part is composed with two parts, the Membrane Anchor 2 and Violacein synthetic pathway enzyme vioA. The enzyme can be aggregated with other membrane anchors, composing the Membrane Magic system.This part is costructed without MS2(BBa_K771112). For more information, please visit 2012 SJTU-BioX-Shanghai wiki

Usage and Biology

In our Violacein Membrane Rudder System, VioA, VioB, VioE and VioC constitutively aggregate. The violacein biosynthetic pathway includes 5 key enzymes which work in conjunction. VioA, a flavoenzyme and VioB, a heme protein, work together to oxidize and dimerize tryptophen into IPA imine dimer. Then vioE induces a indole rearrangement,producing prodeoxyviolacein acid, also known as PVA.

When blue light is present, VioD with Membrane Anchor 6 would aggregate with complex of VioA,B,C and E, making violacein the dominnant final product.But if you turn the light off, VioD with Membrane Anchor would dissociate with VioA,B,C and E. Thus the biosynthetic pathway for deoxyviolacein is switched on.

Related Parts

VioA with Membrane Anchor 2 (BBa_K771201)

VioB with Membrane Anchor 3 (BBa_K771202)

VioE with Membrane Anchor 4 (BBa_K771203)

VioC with Membrane Anchor 5 (BBa_K771204)

VioC with Membrane Anchor 5 (BBa_K771205)

VioD with Membrane Anchor 6 (BBa_K771206)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 1072
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 2310
  Illegal PstI site found at 1072
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 1072
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 1072
  Illegal AgeI site found at 883
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 504
  Illegal SapI.rc site found at 1170