Coding

Part:BBa_K771102

Designed by: GUO Huaqing;SUO Yang;WU Yuqi   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-23)

Lgt with Linker FL3

Inner membrane protein of E.coli

Usage and Biology

To avoid toxicity caused by foreign membrane proteins, we chose phosphatidylglycerol:: prolipoprotein diacylglyceryl transferase (Lgt) as transmembrane domain in the project. Lgt is an inner membrane protein of E.coli with seven transmembrane segments and has been successfully over expressed in E. coli without causing harm to cells. To provide continence to the upperstream protein fusion, a flexible linker is added to the upperstream of lgt.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 805
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 426


[edit]
Categories
Parameters
n/aLgt with Linker FL3