Part:BBa_K770001
LuxCDABE
In pSB1C3 backbone
Comprised of 5 Genes: -LuxA and LuxB codes respectively 40kDA and 37 kDA polypeptides, which together make up the heterodimer bacterial luciferase. -Luciferease requires fatty aldehydes, molecular oxygen -O2 and reduced flavin mononucleotide -FMNH2 as substrates to produce blue-green light emission at 490nm, the reaction products are fatty acid, H2O and flavin mononucleotide -FMN. Lux D: encodes transferase wich transfers Acyl-doners from the fatty acid synthetic pathway by linkage at its serine hydroxyl groups,through hydrolysis, transforming Acyl-doners to a fatty acid. Lux E: encodes Synthetase which with ATP converts the fatty acid to a Acyl-AMP intermediate, which is highly reactive with the cysteine of Synthetase, forming an ester-thiol bond, releasing AMP. Lux C: encodes reductase, which breaks the thiol-ester bond with NADPH to release Fatty Aldehyde. -Together the Lux C, D and E: encodes a Fatty Acid Reductase Enzyme complex which converts acyl-doners to fatty acid then fatty aldehydes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 5274
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2931
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1311
Illegal SapI site found at 3450
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