Composite

Part:BBa_K769021

Designed by: Tomomi YOKOYAMA   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

lux operon(K769011) + luxY(K360100)

Promoter(Pbad)-lux operon-DT + luxY(K360100)


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What is luxY?

Bacterial luciferase derived from Vibrio fischeri, which is a strain of luminous bacteria, shows brue-green light emission (wavelength is 495 nm).
However, Vibrio fischeri strain Y-1 emits longer wavelength light than normal Vibrio fischeri (535 nm, yellow)(a). The yellow shift of light emission wavelength is caused by YFP, which is a secondary emitter protein. YFP does not emit light itself, but it interacts with the luciferase, resulting in a shift of wavelength of light emission. YFP has FMN as a chromophore, and its size is about 28 kDa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1680
    Illegal NheI site found at 5955
    Illegal NheI site found at 8546
    Illegal NheI site found at 8569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6417
    Illegal BamHI site found at 1144
    Illegal XhoI site found at 3767
    Illegal XhoI site found at 4275
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1965
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1859
    Illegal BsaI.rc site found at 2541
    Illegal SapI site found at 961


[edit]
Categories
Parameters
n/alux operon(K769011) + luxY(K360100)