Composite

Part:BBa_K769014

Designed by: Mayumi Nakamura   Group: iGEM12_Tokyo-NoKoGen   (2012-09-19)

Promoter(Pbad)-RBS-luxD-DT

Promoter(Pbad)-RBS-luxD-DT
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This part consists of luxD gene from photobacterium phosphoreum NCMB844. It is placed under an arabinose inducible promoter, Pbad. It is an intermediate construct for making BBa_K769017, with a purpose of making the bioluminescent reaction time shorter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None