Part:BBa_K769011
Promoter(Pbad)-RBS-lux operon-DT
Promoter(Pbad)-RBS-lux operon-DT (Lux operon from Photobacterium phosphoreum NCMB844)
This BioBrick part is constructed by adding arabinose inducible promoter Pbad, RBS and terminator, to our construct BBa_K769010. We cloned this lux operon from Photobacterium phosphoreum NCMB844, which is a marine bioluminescent bacteria. Becasue it is a marine species, it is known to adapt to low temperatures, 20 degrees celcius.
To find out whether our construct expressed inside E. coli will function better at low temperatures or higher temperatures, we cultured the transformed E. coli under two conditions - 20 degress and 30 degees celcius.
The figure below is our result. Non-induced strains, orange and green plots designated as (Ara-), showed no bioluminescent. A red plot, which is a culture that was cultured under 30 degrees celcius with arabinose induction, showed bioluminescent. On the other had, a blue plot, which is a culture that was induced and cultured under 20 degrees celcius showed almost a 5 times higher bioluminescent than the strain cultured under 20 degrees celcius.
The flask in the middle in the image below shows our E. coli culture in a flask, which contains our construct containing the lux operon under Pbad promoter.
The flask on the left: BBa_K769022, middle: BBa_K769011, right: BBa_K769020.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 1680
Illegal NheI site found at 5955 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 6417
Illegal BamHI site found at 1144
Illegal XhoI site found at 3767
Illegal XhoI site found at 4275 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
Illegal AgeI site found at 1965 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1859
Illegal BsaI.rc site found at 2541
Illegal SapI site found at 961
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