Device

Part:BBa_K766007

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-24)


Plas and cI hybrid promoter with RFP

This device is composed of a Plas/cI hybrid promoter and a translational unit of RFP. The Plas/cI hybrid promoter is made up of Plas promoter and cI operator. The promoter can be activated by the LasR-C12HSL complex and repressed by cI repressor. This part can be used in building some logic gates.

The RFP segment is used as a signal to show the result of activation or repression. This kind fluorescent protein is very easy to be detected even by eyes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 57
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 744
    Illegal AgeI site found at 856
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None