Device

Part:BBa_K766006

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-24)


AHL activated Plux-LacI

This device is a protein-expression unit which contains a Plux promoter and a transcriptional unit of LacI without LVA-tag. Plux can be activated by luxR-AHL complex and the promoter can activate the expression of downstream genes. We add LacI in this part. As we all know, LacI is a repressor which can bind with Lac operator. With the help of this feature, many logic gates or gene expression modulation can be achieved.

There is not LVA-tag in this part so that the repressor can keep working for a relatively long time.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None