Reporter

Part:BBa_K766003

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-22)


C6HSL sensor and reporter

We build this part to work as a receiver. C6HSL from the neighbor cell can diffuse across the membrane and activate the transcription from Plux promoter. There is something wrong with original Part:BBa_J09855. So we rebuild the reporter part which can be activated by AHL and add the reporter part RFP which is easily detectable. The expression of it is very clear.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1046
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1046
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1046
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1046
    Illegal AgeI site found at 1632
    Illegal AgeI site found at 1744
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
None