Signalling

Part:BBa_K766001

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-22)


C6HSL reporter

We build this part to work as a receiver. C6HSL from the neighbor cell can diffuse across the membrane and activate the transcription from Plux promoter. We add RFP downstream of the promoter. This kind of RFP protein is easily detectable. The expression of it is very clear.

Compared with the previous design, this part is very convenient. If you need to detect AHL, you just add one drop to the medium. Even the very low concentration of AHL can make the medium turn red very obviously.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


[edit]
Categories
Parameters
None