Device

Part:BBa_K766000

Designed by: Yu Zhao   Group: iGEM12_Tsinghua   (2012-09-22)

AHL synthetase LuxI with His-tag

This part consists of LuxI synthase with His-tag at the N-terminal of the protein. With the help of His-tag, you can detect the expression of this enzyme. Besides, you can purify the protein using Ni-colume and gel filtraion. As a result, you can build a system to product C6HSL in vitro. Between the His-tag and protein, we add the cutting site of thrombin. So that you can cut off the his-tag from the protein.

Besides, you can use Hig-tag antibody to detect the protein in western blot. This design make the detection of luxI gene become very easy.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aAHL synthetase LuxI with His-tag