Device

Part:BBa_K764033

Designed by: Frederic Dudbridge   Group: iGEM12_Exeter   (2012-09-21)

Tetr promoter_rbs_ompA-sacB-Term

A full construct of TetR promoter_rbs_SacB and (double) terminator (BBa_B0014) of Newcastle iGEM 2010's Bio-Brick BBa_K322921 using 3A assembly. The function of SacB remains unperturbed. This was linked to the signal peptide OmpA for export from the cell to prevent damage to Escherichia coli

.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 82
    Illegal SpeI site found at 165
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
    Illegal SpeI site found at 165
    Illegal NotI site found at 88
    Illegal NotI site found at 172
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 82
    Illegal SpeI site found at 165
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 82
    Illegal XbaI site found at 97
    Illegal SpeI site found at 165
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None