Coding

Part:BBa_K764002

Designed by: Frederic Dudbridge   Group: iGEM12_Exeter   (2012-09-15)

WbnK

Encodes the fucosyltransferase wbnK that adds a branched α-L-Fucose donor sugar onto a β-D-Galactose acceptor sugar to form a 1->2 glycosidic bond. The protein wbnK has strict selectivity for Gal-β-(1->3)-GalNAc and acceptor specificity assays have shown that a change of glycosidic bond from 1->3 to 1->4 or from the β to the α configuration both lead to poor substrates for wbnK. wbnK is involved in the formation of both the O86 O-antigen and human blood group B antigen tetrasaccharide. It's close relatives include: wbwK from Escherichia coli O86:B7, wbgL from Escherichia coli O126, wbgN from Escherichia coli O55:H7 and wbgN from Escherichia coli O55:H6.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None