Coding

Part:BBa_K762001

Designed by: Menuka Samaranayake   Group: iGEM12_Wisconsin-Madison   (2012-09-24)

TCC Negative control

This translational coupling cassette uses a version of UW-Madison's codon-optimized LIMS1 gene, (BBa_K762100,) with a stop codon in the middle of the sequence. When this gene is cloned upstream of the translational coupling cassette, the stop codon will prevent the helicase activity of the ribosome from breaking the mRNA hairpin, preventing translation of the red fluorescent protein (RFP) reporter gene. Negative control target gene for the translational coupling cassette made by UW-Madison 2012's team.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1677
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2327
    Illegal AgeI site found at 2439
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 162


[edit]
Categories
Parameters
None