Coding

Part:BBa_K759014

Designed by: Kenta Mine   Group: iGEM12_HokkaidoU_Japan   (2012-09-25)


Codon optimized antigen43 (Ag43, agn43) for Escherichia coli K-12 strain

This Ag43 protein coding site was derived from E. coli K-12 strain and it's codons are optimized to remove 6 PstI recognition sites that it originally had, and for being adapted to E. coli K-12 strain codon usage. E. coli cell-to-cell auto-aggregation would be improved (particularly about expression of this protein) by this optimization.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 49
    Illegal AgeI site found at 259
    Illegal AgeI site found at 295
    Illegal AgeI site found at 493
    Illegal AgeI site found at 568
    Illegal AgeI site found at 607
    Illegal AgeI site found at 967
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None