Composite

Part:BBa_K759013

Designed by: Kenta Mine   Group: iGEM12_HokkaidoU_Japan   (2012-09-20)


P3HB + eYFP producing construct

This is an construct for producing P3HB and eYFP (enhanced yellow fluorescent protein). A constitutive promoter(BBa_R0040) locates on the upstream of the whole construct and by adding 2 percent gulcose into the media, the bacteria would produce bioplastics and eYFP. eYFP exists on the downstream of P3HB production operon for estimating a relationship between amount of produced bioplastics and expressed bioplastic producing enzymes .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 287
    Illegal NgoMIV site found at 402
    Illegal NgoMIV site found at 636
    Illegal NgoMIV site found at 948
    Illegal NgoMIV site found at 1227
    Illegal NgoMIV site found at 1640
    Illegal NgoMIV site found at 1707
    Illegal AgeI site found at 375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3645


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Categories
Parameters
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