Translational_Unit
Part:BBa_K759009
Designed by: Ami Kawahata Group: iGEM12_HokkaidoU_Japan (2012-09-20)
RBS-phaC-RBS-phaA-RBS-phaB
This is a construct of an P3HB (bio plastic) producing operon. RBS(BBa_b0034) is assembled on the upstream of phaC, phaA, phaB gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 225
Illegal NgoMIV site found at 340
Illegal NgoMIV site found at 574
Illegal NgoMIV site found at 886
Illegal NgoMIV site found at 1165
Illegal NgoMIV site found at 1578
Illegal NgoMIV site found at 1645
Illegal AgeI site found at 313 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3583
[edit]
Categories
Parameters
//function/biosynthesis/plastic
None |