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Part:BBa_K755001

Designed by: Krystle McMinn   Group: iGEM12_GeorgiaState   (2012-10-11)


Standardized multiple cloning site

The GSU standardized multiple cloning site (sMCS-1) is a short segment of double stranded DNA that can be customized to modify most commercially available vectors for insertion of iGEM biobricks. This linker includes the standard iGEM multiple cloning sites, in the appropriate order, with an Nhe1 site at the 3' end, to allow insertion at an existing Xba1 site and conversion of the Xba1/Nhe1 overlap into an uncut scar.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 11
    Illegal XbaI site found at 34
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 11
    Illegal NheI site found at 84
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
    Illegal NotI site found at 22
    Illegal NotI site found at 60
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 11
    Illegal XbaI site found at 34
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 11
    Illegal XbaI site found at 34
    Illegal SpeI site found at 49
    Illegal PstI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]


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