Part:BBa_K750113

pBADcIT-PcIGLT: Pbad->cI(lva)->PcI->GFP(lva)

This part contains pBADcIT(BBa_K750104) and PcIGLT(BBa_K750103). Arabinose can activate the promoter pBAD and start up expression of cI protein. Protein cI will activated promoter PcI to repress the expression of GFP(lva). We use this part to make a bright cell turn to dark, to meet demand of number switching. Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1795