Part:BBa_K750106

pBADcIT-0.3:cI protein expression system activated by arabinose(RBS strength:0.3)

This part contains promoter pBAD, RBS of 0.3 strength, cI(lva) protein and double terminator. When arabinose is added, the promoter pBAD will be activated and cI(lva) protein will be produced. cI protein can activated promoter Pci, and repress the production of reporter gene behind it. In our project, sometimes we need the cell can be switched from light to dark, so we designed PciGLT(BBa_K750103), a GFP expression system which can be repressed by cI protein. And this part can provide cI protein for PcIGLT.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]