Signalling

Part:BBa_K750006

Designed by: Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu   Group: iGEM12_XMU-China   (2012-09-18)

LuxR expression device activated by arabinose

This part contains pBAD promoter, rbs of 1.0 strength, LuxR producer and double terminator. Once the promoter pBAD is activated by arabinose, this part can produce LuxR protein. The LuxR protein can bind AHL, which synthesized by LuxI protein. AHL-LuxR can activated the promoter LuxPR, and finally leads to expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
controlK206000
device_typesignalling
proteinsLuxR