Signalling
Part:BBa_K750006
Designed by: Sifan Wang,Ruosang Qiu,Jianzhao Chi,Muxin Yu Group: iGEM12_XMU-China (2012-09-18)
LuxR expression device activated by arabinose
This part contains pBAD promoter, rbs of 1.0 strength, LuxR producer and double terminator. Once the promoter pBAD is activated by arabinose, this part can produce LuxR protein. The LuxR protein can bind AHL, which synthesized by LuxI protein. AHL-LuxR can activated the promoter LuxPR, and finally leads to expression of GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
control | K206000 |
device_type | signalling |
proteins | LuxR |