Part:BBa_K749015

formate dehydrogenase device

Constant protein generator of formaldehyde dehydrogenase 4 (FDH4) from Methylobacterium extroquens.
FDH4 is composed of α-subunit and β-subunit. This parts consists both of them.

Decompose quontify

only 2mM formate in medium
Peak = 8.81(min)

BBa_K749015

Reaction time	volume	Peak area	Peak area(%)
30min	50μl	229371.2	80.88
10min	20μl	248294.2	83.9823

WT

Reaction time	volume	Peak area	Peak area(%)
10min	20μl	232791.6	83.691

No enzym

Reaction time	volume	Peak area	Peak area(%)
10min	20μl	243418.6	84.37

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 2297
Illegal PstI site found at 2009
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 2297
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 2009
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 2297
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 2297
Illegal PstI site found at 2009
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 2297
Illegal PstI site found at 2009
Illegal NgoMIV site found at 1245
Illegal NgoMIV site found at 1508
Illegal NgoMIV site found at 1696
Illegal NgoMIV site found at 1736
Illegal NgoMIV site found at 1866
Illegal NgoMIV site found at 2246
Illegal NgoMIV site found at 2497
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 283
Illegal BsaI site found at 1573
Illegal BsaI.rc site found at 419
Illegal BsaI.rc site found at 1112
Illegal BsaI.rc site found at 1916
Illegal BsaI.rc site found at 2434
Illegal BsaI.rc site found at 2611
Illegal SapI site found at 559
Illegal SapI.rc site found at 121