Coding

Part:BBa_K749005

Designed by: Takuya Akutsu   Group: iGEM12_TMU-Tokyo   (2012-09-26)

PFDH, glutathion-independent formaldehyde dehydrogenase from Pseudomonas putida

PFDH, glutathion-independent formaldehyde dehydrogenase from Pseudomonas putida.
E. coli also has formaldehyde dehydrogenase, but this gene is superior to E. coli's one in following points.

・Glutathion-independence
・Irreversible reaction


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 938
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 938
    Illegal NotI site found at 293
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 938
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 938
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 358
    Illegal NgoMIV site found at 583
    Illegal NgoMIV site found at 880
    Illegal AgeI site found at 211
    Illegal AgeI site found at 397
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 123


[edit]
Categories
Parameters
None