Reporter

Part:BBa_K748005

Designed by: Lei Qiao   Group: iGEM12_HIT-Harbin   (2012-09-15)


P2+RBS+GFP+T. P2 can be activated by phosphorylated AgrA.

P2 can be activated by phosphorylated AgrA, leading to the production of GFP and 3OC6HSL. Thus we can detect the presence of S.aureus expediently by observing the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 765


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter
//function/reporter/fluorescence
Parameters
None