Part:BBa_K747097

Prokaryotic_expression_plasmid

All ccdb parts have been withdrawn from the Registry. Samples of parts containing the ccdB gene cannot be requested. - iGEM HQ

Although for the most part, TAL effectors have been used in eukaryotic organisms, we wanted to enable future iGEM teams to also use this exciting technology in bacteria. So we used BioBrick assembly to construct the following protein generator using the TAL ORF, an IPTG inducible promoter with RBS and a double terminator:
The IPTG inducible promoter with RBS was digested with EcoRI and PstI, the Tal ORF with XbaI and PstI. The two fragments were purified via gel electrophoresis and ligated into pSB1C3 , which was digested with EcoRI and PstI.
The double terminator was digested with XbaI and PstI and the IPTG promoter, TAL ORF fusion construct was digested with EcoRI and PstI. The two fragments were purified via gel electrophoresis and ligated into pSB1A3 , which was digested with EcoRI and PstI.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 798
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1566
  Illegal BamHI site found at 869
  Illegal BamHI site found at 1572
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]